Epitopes of peptide 43-88 of guinea pig myelin basic protein: localization with monoclonal antibodies.
- 1 May 1983
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 130 (5), 2180-2182
- https://doi.org/10.4049/jimmunol.130.5.2180
Abstract
Two monoclonal antibodies, one raised by immunization with mouse myelin basic protein (MBP) and the second raised by immunization with peptide 68-88 of guinea pig MBP, were compared with respect to specificity. The former antibody (15.32) cross-reacted completely with rat, guinea pig, human, and bovine MBP. It also reacted with peptide 43-88 from each MBP. The latter antibody (22.17) was nonreactive with MBP, but cross-reacted with peptide 43-88 from rat, human, guinea pig, and bovine MBP. When tested with small peptides derived from peptide 43-88, antibody 22.17 reacted with an epitope in the C-terminal region. Antibody 15.32 reacted with an epitope in the N-terminal half of the peptide. The data show that 22.17 reacted with a unique epitope associated only with free peptide, whereas 15.32 recognized an epitope common to both peptide 43-88 and MBP.This publication has 4 references indexed in Scilit:
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