A SPECIFIC STAINING FOR NUCLEIC ACIDS WITH TOLUIDIN BLUE?

Abstract

Sections of spinal cord were stained with toluidin blue at pH 2.2 for 2 min. and differentiated for 18 hours in a mixture of equal vols. of n-butyl alcohol and ethanol. The staining seemed to be specific for nucleic acids, as tested through incubation with ribonuclease and control buffer solutions Specific stainings -could not be obtained with a gallocyanin-chromalum.