THE SARCOSINE OXIDASE IN ADAPTED AND UNADAPTED CULTURES OF A STRAIN OF PSEUDOMONAS AERUGINOSA

Abstract

The sarcosine oxidase in a strain of Pseudomonas aeruginosa is increased by growing the organisms in media containing creatinine. There is no corresponding increase in the enzymes that oxidize glycine, formaldehyde, and formic acid, the oxidation products of sarcosine. Comparison of the actions of certain amidines and a detergent on the adapted and unadapted cells indicates that the extra sarcosine oxidase in the former is in or near the cell surface, whereas the constitutive enzyme is in the cell interior.