Endogenous ADP‐ribosylation of elongation factor 2 in polyribosome fraction of rabbit reticulocytes
- 15 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (1), 261-263
- https://doi.org/10.1016/0014-5793(84)80953-9
Abstract
Several polypeptides of about 120, 96, 85, 60 and 38 kDa are shown to be radiolabeled during incubation of the mono- and polyribosome fraction of rabbit reticulocytes with [ 32 P]NAD. Among them is a polypeptide coinciding with elongation factor 2 (EF-2) in its electrophoretic mobility in SDS-polyacrylamide gel. The addition of pure EF-2 to the polyribosome fraction results in an increase of the radioactive label in this polypeptide band. From this it is concluded that both endogenous and added EF-2 is ADP-ribosylated by an enzyme associated with polyribosomes. A possibility of regulation of protein synthesis through endogenous ADP-ribosylation in vivo is considered.Keywords
This publication has 14 references indexed in Scilit:
- Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A.Proceedings of the National Academy of Sciences, 1984
- Adenosine diphosphate ribosyltransferase and protein acceptors associated with cytoplasmic free messenger ribonucleoprotein particlesBiochimie, 1984
- (ADP-ribose)n participates in DNA excision repairNature, 1980
- Posttranslational modification of elongation factor 2 in diphtheria-toxin-resistant mutants of CHO-K1 cells.Proceedings of the National Academy of Sciences, 1980
- Isolation of an avian erythrocyte protein possessing ADP-ribosyltransferase activity and capable of activating adenylate cyclaseProceedings of the National Academy of Sciences, 1978
- Cytoplasmic poly(ADP-ribose) polymerase during the HeLa cell cycleArchives of Biochemistry and Biophysics, 1975
- Interrelationship between poly (ADP-Rib) synthesis, intracellular NAD levels, and muscle or cartilage differentiation from mesodermal cells of embryonic chick limb.Proceedings of the National Academy of Sciences, 1975
- Purification and properties of rabbit reticulocyte protein synthesis elongation factor 2.Journal of Biological Chemistry, 1975
- Poly ADP-ribose synthesis and DNA replication in synchronized mouse L-cellsBiochemical and Biophysical Research Communications, 1973
- Factors affecting the rate of protein synthesis in lysate systems from reticulocytesArchives of Biochemistry and Biophysics, 1968