Effet de l’insuline et de quelques autres facteurs sur la glycolyse des leucocytes humains mesurée in vitro

Abstract

Leukocyte suspensions were prepared from human non-diabetic subjects and their aeorbic metabolic activity measured in vitro under various conditions. No crowding effect was noted in the glucose consumption or in the lactic acid production. In absence of added glucose, lactic acid was produced and intra-cellular glycogen diminished; when the medium contained glucose the lactic acid formation increased and intracellular glycogen was synthesized; this lactic acid production and glycogen synthesis did not vary as a function of the glucose concentration in the medium. The glucose consumption depends partially on the glucose concentration in the medium. Leukocytes from fasting subjects and leukocytes from subjects in a state of hyperglycemia and hyperinsulinemia showed the same glucose consumption and the same lactic acid production. In a medium containing autologue serum, the lactic acid production, but not the glucose consumption, was strongly stimulated. In vitro, neither the glucose consumption, nor the lactic acid production were affected by addition of insulin. These results are discussed in relation to the regulation of the leukocyte metabolism.