Transcription in Yeast: A Factor that Stimulates Yeast RNA Polymerases
- 1 October 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (10), 2818-2822
- https://doi.org/10.1073/pnas.69.10.2818
Abstract
Yeast cells contain an RNA polymerase factor, pi, which is a heat-stable protein with an apparent molecular weight of 12,000. This factor stimulates transcription of calf-thymus, salmon-sperm, yeast-nuclear, and T4-phage DNA. It stimulates transcription by each of the four yeast-nuclear RNA polymerases, by rat-liver RNA polymerases I and II, and by Escherichia coli RNA polymerase. pi-Factor can cause each of the eukaryotic RNA polymerases to become insensitive to rifamycin AF-013, but does not stop inhibition of E. coli RNA polymerase by rifamycin AF-013. Stimulation of transcription by pi-factor is general, and does not apply only to a limited class of genes. Apparently, pi-factor stimulates transcription by increasing the proportion of RNA polymerase binding events that leads to the initiation of RNA chains.Keywords
This publication has 15 references indexed in Scilit:
- DNA‐dependent RNA polymerases from yeast. Partial characterization of three nuclear enzyme activitiesFEBS Letters, 1971
- The Regulation of Phage DevelopmentAnnual Review of Microbiology, 1970
- Multiple Forms of DNA-dependent RNA Polymerase in Eukaryotic OrganismsNature, 1969
- Breaks in DNA stimulate Transcription by Core RNA PolymeraseNature, 1969
- RNA transcribed from reconstituted nucleoprotein is similar to natural RNAJournal of Molecular Biology, 1969
- Factor Stimulating Transcription by RNA PolymeraseNature, 1969
- The Biology of Isolated ChromatinScience, 1968
- The binding of RNA polymerase to DNAJournal of Molecular Biology, 1966
- Selective synthesis of T-even bacteriophage early messenger in vitroJournal of Molecular Biology, 1966
- HISTONE, A SUPPRESSOR OF CHROMOSOMAL RNA SYNTHESISProceedings of the National Academy of Sciences, 1962