Automatic column chromatographic analysis of saccharides and uronic acids

Abstract

Using n-proponal/water as the eluting solvent, a flow rate of 50 ml/h and a 90°C column temperature, the separation of a complex mixture of 11 monosaccharides could be much improved over the procedure described by v. Wilm and Sordé using isopropanol/water, 70–100 ml/h flow rate and temperatures of 83–90°C. With a small total error of 3%, the saccharide analysis lies in the region of reproducibility that has also been reached in amino acid analysis. The limit of detection for saccharides lies at 1 × 10⁻⁹ mole/ml.