Structural characterization and expression of a brain specific gene encoding chick kainate binding protein
- 10 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 297 (3), 257-262
- https://doi.org/10.1016/0014-5793(92)80551-q
Abstract
The gene encoding chick kainate-binding protein (c-KBP), a member of the non-NMDA ionotropic glutamate receptor family has been isolated and characterized. The c-KBP gene is at least 13 kilobases long and contains 11 exons interrupted by 10 introns. Primer extension and RNase protection studies identified a major transcription initiation site located 117 bases upstream from the initiation methionine codon ATG. Consensus TATA and CCAAT sequences were detected in the putative promoter region. The structure of the c-KBP gene is strikingly different from that of other members of neurotransmitter-gated ion-channels (cloned at present) although the topology of c-KBP consists of four membrane-spanning domains, a structural characteristic of ionotropic receptor subunits. The c-KBP gene was found to be expressed at high levels in chick cerebellar Bergmann glia and at extremely low levels in the forebrain. The limited expression of the c-KBP gene raises important questions concerning the mechanisms governing the regulation of c-KBP gene transcription.Keywords
This publication has 24 references indexed in Scilit:
- New wave of non-NMDA excitatory amino acid receptorsTrends in Pharmacological Sciences, 1991
- Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptorsTrends in Pharmacological Sciences, 1991
- Conserved Organization of ?-Aminobutyric AcidAReceptor Genes: Cloning and Analysis of the Chicken ?4-Subunit GeneJournal of Neurochemistry, 1991
- Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cellsNature, 1991
- Flip and Flop: A Cell-Specific Functional Switch in Glutamate-Operated Channels of the CNSScience, 1990
- A Family of AMPA-Selective Glutamate ReceptorsScience, 1990
- Molecular structure of the chick cerebellar kainate-binding subunit of a putative glutamate receptorNature, 1989
- Sequence and expression of a frog brain complementary DNA encoding a kainate-binding proteinNature, 1989
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981