Studies on Pituitary Follitropin. Iv. A Conformation Specific Radioimmunoassay for the Ovine Hormone
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 5 (4), 279-291
- https://doi.org/10.1080/07435807809061093
Abstract
An antiserum to partially purified ovine follitropin (50 x NIH-FSH-S10) shows species specificity. It is conformation dependent and requires the proper recombination of the α and β subunits for maximal reactivity. The isolated α subunit is essentially inactive and the hormone specific β subunit is weakly reactive. The homologous radioimmunoassay is valuable for estimating native ovine follitropin in the presence of free subunits. It also provides a sensitive method to study association-dissociation and structure-function relationships.Keywords
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