Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy

Abstract

Objective(s) The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal´s welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for Boron Neutron Capture Therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and Methods We studied: Classical cancerization protocol (24 applications); Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results The twice‐interrupted group exhibited a significantly lower percentage of animals with severe mucositis vs the non‐interrupted group (17% vs 71%) and a significantly higher incidence of long pouches (100% vs 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT‐induced severe mucositis. Conclusion(s) The twice‐interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favoured the animal’s welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.