Construction of Mouse A9 Clones Containing a Single Human Chromosome (X/Autosome Translocation) via Micro‐cell Fusion

Abstract

Cell hybrids between hypoxanthine guanine phosphoribosyl transferase (HGPRT)‐deficient mouse cell lines (A9 or RAG) and each of 12 different human flbroblasts (GM cells) containing various X/autosome translocations were formed, selected and isolated. Several human chromosomes including an X/autosome translocation carrying HGPRT locus were found in these hybrid cells. To construct A9 cell clones that contain a single X/autosome translocation, micro‐cell fusion was undertaken to transfer these chromosomes from the hybrids to A9 cells. Karyotype analysis revealed that most of the resulting micro‐cell hybrids contain, in a background of mouse chromosomes, only the human X/autosome translocations which were present in the GM cells used for cell hybridization. Sublines of A9 cells were established containing the following autosomal segments: 1q23→1qter; 1q12→1pter; 3p12→3pter; 3q21→3qter; 11q13→11qter; 11q13→11pter; 11p11→11qter; 11q23→11pter; 12q24→12pter; 16q24→16pter; 17q11→17pter.