Dissociation-recombination of intergenic sunflower alcohol dehydrogenase isozymes and relative isozyme activities
- 1 February 1976
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 14 (1-2), 87-98
- https://doi.org/10.1007/bf00484876
Abstract
Two unlinked genes, Adh 1 and Adh 2, control the production of alcohol dehydrogenase (ADH) in seeds of the annual sunflower (Helianthus annuus). Each gene is polymorphic, having F and S alleles. Starch gel electrophoretic zymograms of the four possible double homozygotes have three bands, representing two homodimers and an intermediately migrating intergenic isozyme. Zymograms of double heterozygotes consist of nine bands produced by ten isozymes: six intragenics and four intergenics, two of which are coincident. Results of dissociation-recombination (D-R) experiments are reported which demonstrate the subunit composition of the intergenic isozymes, thus supporting the relationships suggested by genetic studies. Densitometric tracings of the zymogram of a cleared gel and measurements of activities of homodimer isozymes eluted from gels following D-R of an intergenic isozyme showed that the Adh 2 isozymes were more than twice as active as those of Adh 1. Measurements of activities of crude extracts from the four possible double homozygous genotypes indicated that the seeds of the genotype Adh 1 F /Adh 1 F , Adh 2 S /Adh 2 S produced more activity than the other three. This genotype is the most common one found in wild and cultivated stocks. Isozymes eluted following electrophoresis of the same extracts had averages of 19%, 70%, and 11% of total activity contributed by the Adh 1, Adh2, and intergenic isozymes, respectively. A simple but efficient method of isozyme elution from starch gels is described which resulted in nearly full expected recovery (approximately 46%) of the ADH activity in the applied sample.This publication has 28 references indexed in Scilit:
- Isozymes in Development and DifferentiationAnnual Review of Plant Physiology, 1974
- Sunflower alcohol dehydrogenase: ADH1 genetics and dissociation-recombinationBiochemical Genetics, 1974
- Genetic relationships between the multiple alcohol dehydrogenases of maizeBiochemical Genetics, 1973
- Differences between maize inbreds in the activity level of the AP1-controlled acid phosphataseBiochemical Genetics, 1971
- Gene duplication in fishes: Malate dehydrogenases of salmon and troutBiochemical and Biophysical Research Communications, 1969
- Genetic control of multiple molecular forms of enzymes in plants: A reviewBiochemical Genetics, 1969
- Isozymes: Classification, Frequency, and SignificanceInternational Review of Cytology, 1969
- NATURE OF MULTIPLE MOLECULAR FORMS OF ENZYMES*Annals of the New York Academy of Sciences, 1968
- AN APPROACH TO THE PROBLEM OF CONFORMATIONAL ISOZYMESAnnals of the New York Academy of Sciences, 1968
- Lactate Dehydrogenase Isozymes: Dissociation and Recombination of SubunitsScience, 1963