Finn chamber patch test technique

Abstract

Proper technique aids reliable patch test results. The Finn chamber volume was determined to be 25 μl. These studies suggest the optimal volume semi‐solid test substance 10 be 12–15 μl. More than 18‐21 μl caused significant extrusion of test substance and improper adhesive adherence. Finn chamber paper discs used with solutions absorb a maximum of 17.5 μl test solutions and keep 15μl within the chamber. The evaporation rate of aqueous test solutions from Finn chambers was 1 mg/3 min; thus, the chambers should be applied quickly to avoid drying.