Na+-dependent transport of pyruvate in erythrocytes of the Pacific hagfish (Eptatretus stouti)
- 1 November 2000
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 78 (11), 2019-2026
- https://doi.org/10.1139/z00-145
Abstract
We investigated the mechanisms of pyruvate transport in the erythrocytes of an ancient marine agnathan, the Pacific hagfish (Eptatretus stouti), and a sedentary euryhaline teleost, the starry flounder (Platichthys stellatus). Uptake of [14C]pyruvate (50 µM, 10°C) by flounder erythrocytes was slow (t1/2(half-life) ~ 30 min), nonconcentrative, and mediated by the band 3 Cl-/HCO-3exchanger in combination with a process similar to the H+/monocarboxylate symporter present in freshwater teleosts and mammalian erythrocytes. In contrast, pyruvate uptake by hagfish erythrocytes (50 µM, 10°C) was rapid (t1/2~ 1.5 min) and, in 10 min, reached an intracellular concentration more than 20-fold higher than that present in the extracellular medium. Pyruvate accounted for almost 90% of the accumulated intracellular radioactivity, the remaining label being incorporated into tricarboxylic acid cycle intermediates and glutamate. Influx of pyruvate was saturable (apparent Km= 12 mM) and inhibited by p-chloromercuriphenylsulphonate (PCMBS) (Ki= 71 µM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonate (DIDS) (Ki= 0.49 mM). Transport was inhibited poorly by α-cyano-4-hydroxycinnamate (CIN) (Ki> 4 mM) and was not coupled to the movement of protons. Instead, the influx of pyruvate was Na+dependent. A sigmoidal relationship between pyruvate transport and extracellular Na+concentration was observed, suggesting a Na+:pyruvate coupling ratio greater than 1:1. In contrast with previously described Na+-dependent monocarboxylate transport activities in mammalian renal and intestinal epithelia, the hagfish erythrocyte system did not transport lactate.Keywords
This publication has 42 references indexed in Scilit:
- Taurine efflux, band 3, and erythrocyte volume of the hagfish (Myxine glutinosa) and lamprey (Petromyzon marinus)Journal of Experimental Zoology, 1992
- Evidence for bumetanide-sensitive, Na+-dependent, partial Na–K–Cl co-transport in red blood cells of a primitive fishCanadian Journal of Physiology and Pharmacology, 1991
- Metabolic energy production during adrenergic pH regulation in red cells of the Atlantic salmon, Salmo salarRespiration Physiology, 1988
- Effect of electroneutral luminal and basolateral lactate transport on intracellular pH in salamander proximal tubules.The Journal of general physiology, 1987
- Volume-sensitive taurine transport in fish erythrocytesThe Journal of Membrane Biology, 1987
- Specificity of the Na+-dependent monocarboxylic acid transport pathway in rabbit renal brush border membranesThe Journal of Membrane Biology, 1983
- The mechanism of Na+-l-lactate cotransport by brush border membrane vesicles from horse kidney: Analysis of rapid equilibrium kinetics in absence of membrane potentialThe Journal of Membrane Biology, 1983
- Monocarboxylate transport in erythrocytesThe Journal of Membrane Biology, 1982
- The membrane permeability of nonelectrolytes and carbohydrate metabolism of Amazon fish red cellsCanadian Journal of Zoology, 1978
- Oxygen uptake by rainbow trout blood, Salmo gairdneriJournal of Fish Biology, 1977