Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14?q15

Abstract

Human × rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14–q15. M6P1 is distinct from NT5, coding for 5′ nucleotidase, which maps to the same region.