Comparison of Reagents for Lipid and Phospholipid Detection and Densitometric Quantitation on Silica Gel and C18Reversed Phase Thin Layers

Abstract

A variety of chromogenic and fluorogenic detection reagents were evaluated for detection and densitometric quantification of lipids and phospholipids on silica gel and chemically bonded octadecylsilane reversed phase thin layer plates (RP-TLC). Phosphomolybdic acid (PMA) was the most generally favorable reagent, followed by cupric acetate and ethanolic sulfuric acid. The quantification of lipids in human blood serum by chemically bonded RP-TLC was demonstrated using PMA with direct sample application to preadsorbent plates.