Determination of the sequences of 18 nucleotides from the 5′-end of the 1-strand and 15 nucleotides from the 5′-end of the r-strand of T7 DNA
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6), 839-852
- https://doi.org/10.1093/nar/2.6.839
Abstract
The sequences of 18 nucleotides from the 5′-end of the 1-strand and 15 nucleotides from the 5′-end of the γ-strand of T7 bacteriophage DNA have been determined to be pT-C-T-C-A-C-A-G-T-G-T-A-C-G-T-C-C-C (1-strand) and pA-G-G-G-A-C-A-C-A-G-C-G-C-T-C (r-strand)*. The 5′-termini of whole DNA or separated strands were kinased using polynucleotide kinase and (γ-32P; rATP. The DNA was partially digested with pancreatic DNase and the fragments were separated by two dimensional electrophoresis and homochromatography. To complete the sequence, snake venom phosphodiesterase digestions of these fragments were carried out. The relationship of these sequences to the proposed cleavage of concatemeric DNA during DNA replication is discussed.Keywords
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