Selection of Auxotrophic Bacterial Mutants by Tritium-Labeled Thymidine
- 27 March 1959
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 129 (3352), 838-839
- https://doi.org/10.1126/science.129.3352.838
Abstract
A method is proposed for the efficient isolation of auxotrophic mutants of cells of diverse origin by the use of radioactive materials. An example is described in which mutants of Escherichia coli B were selected by growing irradiated cells in the presence of tritium-labeled thymidine.Keywords
This publication has 11 references indexed in Scilit:
- Inhibition of HeLa Growth by Intranuclear TritiumScience, 1958
- Distribution of Tritium-Labelled Thymidine in Escherichia Coli During Cell MultiplicationNature, 1958
- Synthesis of nucleic acids in ultraviolet-treated Escherichia coliBiochimica et Biophysica Acta, 1957
- STUDIES OF DEOXYRIBONUCLEIC ACID BIOSYNTHESIS IN EMBRYONIC TISSUES WITH THYMIDINE-C14Journal of Biological Chemistry, 1956
- ROLE OF VITAMIN B12 IN NUCLEIC ACID METABOLISMJournal of Biological Chemistry, 1956
- ROLE OF VITAMIN-B-12 IN NUCLEIC ACID METABOLISM .4. METABOLISM OF C-14-LABELED THYMIDINE BY LACTOBACILLUS LEICHMANNII1956
- THE SEPARATION AND ISOLATION OF PARTICULAR BIOCHEMICAL MUTANTS OF NEUROSPORA BY DIFFERENTIAL GERMINATION OF CONIDIA, FOLLOWED BY FILTRATION AND SELECTIVE PLATINGProceedings of the National Academy of Sciences, 1954
- REPLICA PLATING AND INDIRECT SELECTION OF BACTERIAL MUTANTSJournal of Bacteriology, 1952
- UTILIZATION OF DESOXYRIBOSIDES IN THE SYNTHESIS OF POLYNUCLEOTIDESJournal of Biological Chemistry, 1951
- MUTANTS OF ESCHERICHIA COLI REQUIRING METHIONINE OR VITAMIN B 12Journal of Bacteriology, 1950