Different Particle Types in Tissue Culture and Intestinal Epithelium Infected with Rotavirus

Abstract

Rotavirus-infected [calf] intestinal epithelial cells in vivo and calf kidney cells in vitro were examined by EM. Morphogenesis takes place in the cytoplasm and several particle types are observed. These can be classified broadly into 2 groups, one of which appears to represent the characteristic normal development of infectious virus and the other, distinctly different, which may be a non-infectious form. Two of the particle types are tentatively identified as the single and double capsid rotavirus particles seen typically in negatively stained preparations.