A Simple Quantitative Test for Cytostatic Agents Using Non-adhering Cells in Vitro

Abstract

The use of P-815 mouse mastocytoma cells in an in vitro test designed for screening potential cytostatic drugs is described. The test is based on the fact that P-815 cells do not attach themselves to the glass and reproduce at a high rate in a conventional medium. Quantitation of growth inhibition is by cell count, performed in an electronic cell counter. Comparison of results obtained by this new method with those produced in other tests demonstrates the high sensitivity of P-815 cells for cytostatic and cytotoxic compounds.