A Correction and Extension of the Acetylated Amino Terminal Sequence of Ovalbumin

Abstract

The acetylpeptides derived from S-carboxymethylovalbumin by cyanogen bromide and chymotrypsin were isolated and shown by enzyme digestion and the dansyl-Edman method to fit the sequence acetyl-Gly-Ser-Ile-Gly-Ala-Ala-Ser-Met-Glu-Phe. This corrects the order of the 3rd and 4th residues in the 5-residue sequence given by Narita and Ishii, 1962. The overlap of the C-terminal sequence of this extended sequence with the 6-residue N-terminal sequence surrounding a half-cystine residue in ovalbumin gives the N-terminal sequence for ovalbumin as acetyl-Gly-Ser-Ile-Gly-Ala-Ala-Ser-Met-Glu-Phe-Cys-Phe-Asp-Val-Phe-Lys with residue 11 a cysteine residue.