The Effect of Transient Dopamine Antagonism on Thyrotropin-Releasing Hormone-Induced Prolactin Release in Ovariectomized Rats Treated with Estradiol and/or Progesterone*

Abstract

PRL release was studied on ovariectomized (OVX) rat pretreated with estradiol benzoate (EB), progesterone (P), or a combination of both steroids using a protocol that was selected to mimic ovarian steroid changes that have been observed during the female rat 4-day estrous cycle and early pregnancy. On the morning of the experiment, the animals received injections of either the dopamine (dA) antagonist domperidone (0.01 mg/rat iv) or vehicle (acetic acid in saline) five minutes later, all animals received injections of the DA agonist 2-bromo-.alpha.-ergocryptine (CB-154; 0.5 mg/rat, iv), followed 60 min later by the administration of TRH (1.0 .mu.g/rat, iv). Plasma obtained from blood samples taken during the experiment was assayed for PRL by RIA. In OVX or P-treated OVX rats, a transient blockade of DA by domperidone did not alter the sensitivity of the pituitary to TRH administration, as measured by an increase in plasma PRL. However, such an effect of DA blockade was induced by 2 days of EB treatment and was maintained and amplified by P administration after EB injections. We conclude that enhancement of the PRL-releasing effect of TRH by DA antagonism, a mechanism we previously observed in female rats during midlactation, proestrus, estrus and metestrus using the present drug protocol, can be induced by estrogen and maintained by P. Further, our data suggest that the previously observed loss of this secretory mechanism on the morning of diestrus may be due to the decrease in plasma P that takes place between metestrus and diestrus.