Absorption of Proteins and Peptides in the Far Ultraviolet
- 9 January 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 167 (3915), 179-181
- https://doi.org/10.1126/science.167.3915.179
Abstract
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6°C to take advantage of the reduction of end-absorption of water with temperature.Keywords
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