Identification of domains of a cloned rat brain GABA transporter which are not required for its functional expression
- 22 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (1), 41-44
- https://doi.org/10.1016/0014-5793(93)81323-r
Abstract
The sodium and chloride coupled γ-aminobutyric acid (GABA) transporter purified from rat brain, belongs to a superfamily of neurotransmitter transporters. They are involved in the termination of synaptic transmission and are predicted to have 12 membrane spanning α-helices with both amino- and carboxyl-termini oriented toward the cytoplasm. In order to define the domains not required for functional expression, we have constructed and expressed a series of deletion mutants in GAT-1, the cDNA clone encoding for the transporter. Transporters truncated at either end until just a few amino-acids distance from the beginning of helix 1 and the end of helix 12, retain their ability to catalyze sodium and chloride-dependent GABA transport.Keywords
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