Heterologous B-Cell Antisera May Detect Non-Ig, Non-HLA-DR Antigens
- 1 July 1979
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 10 (1), 17-24
- https://doi.org/10.1111/j.1365-3083.1979.tb01329.x
Abstract
A heterologous antiserum (antiserum 7420) against B-lymphocyte antigen(s) was raised in a rabbit by immunization with peripheral blood lymphocytes from a patient with chronic lymphocytic leukaemia (CLL). After absorptions with pooled normal human serum, IgA, IgD, and IgM M-components, the fluorescein isothiocyanate-conjugated F(ab′)2 fragments were prepared and further absorbed with normal peripheral blood leucocytes. The F(ab′)2 fragments, studied in direct immunofluorescence, reacted with both normal and CLL B lymphocytes but not with T lymphocytes. Comparative studies with an HLA-DR antiserum showed that the antigen(s) detected by 7420 antiserum did not redistribute together with HLA-DR antigens in cocapping experiments, nor did the HLA-DR antiserum block the reaction of 7420 F(ab′)s fragments with B lymphocytes. The 7420 F(ab′)z fragments precipitated detergent-solubilized B-cell membrane material with a molecular weight of around 40,000 and 150,000 daltons. The conclusion drawn is that the 7420 and HLA-DR antigens are different. The 7420 antigen was also shown to be different from classical HLA antigens, β2-microglobulin, surface immunoglobulin, the Fc receptor, and HC protein.This publication has 29 references indexed in Scilit:
- Reactivity of a Rabbit Antiserum against Highly Purified HLA‐DR AntigensScandinavian Journal of Immunology, 1978
- Two‐Chain Subunit Structure of HLA‐D AntigensTissue Antigens, 1977
- Human B cell line deficient in the expression of B cell‐specific glycoproteins (GP 27,35)European Journal of Immunology, 1977
- Cellular Distribution, Purification, and Molecular Nature of Human la AntigensScandinavian Journal of Immunology, 1977
- The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization.The Journal of Experimental Medicine, 1976
- Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera.The Journal of Experimental Medicine, 1976
- Presence of Chronic Lymphoid Leukaemia Associated Antigen in Other Malignant Haematological DiseasesVox Sanguinis, 1974
- Antigens Specific for Human Lymphocytic and Myeloid Leukemia Cells: Detection by Nonhuman Primate AntiserumsScience, 1972
- Detection of an Antigen Associated with Acute LeukemiaScience, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970