Plasmid-mediated Chloramphenicol Resistance in Staphylococcus hyicus
- 1 December 1989
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 135 (12), 3329-3336
- https://doi.org/10.1099/00221287-135-12-3329
Abstract
A small plasmid of 3.95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 Staphylococcus hyicus strains. The plasmid in each of the three strains was indistinguisable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.This publication has 4 references indexed in Scilit:
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