Genetic instability at the adenine phosphoribosyltransferase locus in mouse L cells.
Open Access
- 1 March 1982
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 2 (3), 250-257
- https://doi.org/10.1128/mcb.2.3.250
Abstract
Resistance to adenine analogs such as 2,6-diaminopurine occurs at a rate of approximately 10(-3) per cell per generation in mouse L cells. This resistance is associated with a loss of detectable adenine phosphoribosyltransferase activity. Other genetic loci in L cells have the expected mutation frequency (approximately 10(-6)). Transformation of L cell mutants with Chinese hamster ovary cell DNA results in transformants with adenine phosphoribosyltransferase activity characteristic of Chinese hamster ovary cells. No activation of the mouse gene occurs on hybridization with human fibroblasts. That this high frequency event is the result of mutation rather than an epigenetic event is supported by antigenic and reversion studies of the 2,6-diaminopurine-resistant clones. These results are consistent with either a mutational hot-spot, a locus specific mutator gene, or a site of integration of an insertion sequence.This publication has 36 references indexed in Scilit:
- Retroviruses as mutagens: Insertion and excision of a nontransforming provirus alter expression of a resident transforming provirusCell, 1981
- Transforming DNA integrates into the host chromosomeCell, 1981
- Transposable elementsCell, 1980
- Reversible inactivation of autosomal alleles in chinese hamster cellsJournal of Cellular Physiology, 1979
- Gene inactivation as a mechanism for the generation of variability in somatic cells cultivated in vitroMutation Research, 1977
- On the nature of hereditable variation in cultured somatic cellsCell, 1976
- Appearance of hypoxanthine guanine phosphoribosyltransferase activity as a consequence of mycoplasma contaminationNature, 1975
- Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detectionMutation Research, 1974
- Mutants of cultured chinese hamster cells deficient in adenine phosphoribosyl transferaseCell, 1974
- A table for the estimation of the spontaneous mutations rate of cells in cultureMutation Research, 1973