Enzyme Immunoassays for Measurement of Cytomegalovirus Immunoglobulin M Antibody
- 1 October 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 14 (4), 427-432
- https://doi.org/10.1128/jcm.14.4.427-432.1981
Abstract
The diagnosis of congenital cytomegalovirus (CMV) infection is often accomplished by the detection of circulating antibody directed against CMV. A method was devised for measuring CMV-specific IgM based on the isolation of IgM antibody by reaction with a solid phase coated with antihuman IgM. The determination of IgM antibody specific for CMV was accomplished by the addition of CMV or control antigen and enzyme-labeled CMV antibody (solid phase-IgM method). The sensitivity and specificity of this method were compared with those of a conventional form of solid-phase enzyme immunoassay in which CMV antigen is bound to the solid phase (solid phase-antigen method). Both assay systems were capable of detecting CMV-specific IgM antibody in the sera of 10 [human] babies with documented CMV infection and in the mothers of 4 of these babies. The solid phase-IgM method yielded negative results in all 66 sera available from babies who did not have congenital CMV infection. The solid phase-antigen system yielded false-positive results in 12 (18%) of these sera and in 8 of 12 sera obtained from patients with demonstrable rheumatoid factor. The solid phase-IgM system yielded negative results for the rheumatoid sera provided that appropriate control reactions were performed. The solid phase-IgM system is thus a specific and sensitive method for the determination of CMV IgM antibody.This publication has 36 references indexed in Scilit:
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