Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.
Open Access
- 1 September 1982
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 70 (3), 568-578
- https://doi.org/10.1172/jci110649
Abstract
The expression of Ia-like antigens on human colony forming units-granulocyte macrophage (CFU-GM) is related to S-phase of the cell cycle, and associated with the control of normal granulocyte and macrophage production by prostaglandin E and acidic isoferritins in vitro. Ia-antigen expression by CFU-GM is lost within 3-6 h of culture at 37 degrees C and occurs simultaneously with loss of responsiveness to inhibition by these factors. Culture of bone marrow CFU-GM in a limited exposure suspension culture with 1 microM-1pM prostaglandin E (PGE1 or PGE2), but not prostaglandin F2 alpha or dibutyryl-cyclic-3'-5'-AMP results in the detection of CFU-GM Ia-antigen after 24 h. Antigen expression is associated with an absolute increase in total and S-phase CFU-GM, and restoration of responsiveness to inhibition by prostaglandin E and acidic isoferritins. The detection of Ia-antigen on CFU-GM after suspension culture with prostaglandin E results both from Ia-antigen reexpression as well as stimulation of noncycling cells to enter S-phase, express Ia-antigen and give rise to CFU-GM sensitive to inhibition by prostaglandin E and acidic isoferritins. The sensitivity of CFU-GM to inhibition by these factors after suspension culture with prostaglandin E is identical to that of the same cells tested prior to the suspension culture. These studies provide evidence for a direct regulatory association between Ia-antigen expression and control of myeloid progenitor cell differentiation, and suggest a role for prostaglandin E in the control of CFU-GM cell cycle, Ia-antigen expression, and growth regulation.This publication has 35 references indexed in Scilit:
- Relationship of Cell-Cycle Expression of Ia-like Antigenic Determinants on Normal and Leukemia Human Granulocyte-Macrophage Progenitor Cells to Regulation In Vitro by Acidic IsoferritinsJCI Insight, 1982
- REGULATION OF HUMAN MYELOPOIESIS BY PROSTAGLANDIN E AND LACTOFERRINCell Proliferation, 1981
- Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells.The Journal of Experimental Medicine, 1980
- Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages.The Journal of Experimental Medicine, 1980
- Hematopoietic Stem CellsNew England Journal of Medicine, 1979
- Regulation of macrophage and granulocyte proliferation. Specificities of prostaglandin E and lactoferrinThe Journal of Experimental Medicine, 1979
- Limitation of Excessive Myelopoiesis by the Intrinsic Modulation of Macrophage-Derived Prostaglandin EScience, 1978
- Antigens expressed by human B lymphocytes and myeloid stem cells.The Journal of Experimental Medicine, 1977
- Prostaglandin E2 as stimulator of haemopoietic stem cell proliferationNature, 1974
- Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human PlasmaJCI Insight, 1973