A fluorescence method for the determination of the molecular weight cut-off of alginate-polylysine microcapsules
- 12 April 1991
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 43 (4), 275-277
- https://doi.org/10.1111/j.2042-7158.1991.tb06683.x
Abstract
Several applications of microcapsules for the encapsulation of living cells or macromolecules require well defined pore sizes. The molecular weight cut-off of alginate-polylysine microcapsules has been determined using a range of fluorescent labelled dextran molecules. The diffusion of the fluorescein isothiocyanate labelled (FITC)-dextrans into the microcapsules was followed by fluorescence and confocal laser scanning microscopy. The permeability of microcapsules for FITC-dextrans with a molecular weight of 4 700 daltons and the impermeability for FITC-dextrans with a molecular weight of 40 500 daltons was confirmed with both techniques. Determination of the molecular weight cut-off, using confocal laser scanning microscopy was more reliable and required a smaller sample than fluorescence measurements.Keywords
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