Über Symplexbildung des Kallikrein-Inhibitors aus Rinderorganen mit Heparin

Abstract

The kallikrein inhibitor from bovine liver can be precipitated by heparin according to the method of Appel and Werle. The inhibitor from bovine lung can also be precipitated by the same method, whereby more than 8 moles of inhibitor becomes bound by 1 mole of heparin. The simplex of heparin and kallikrein inhibitor is soluble in very dilute NaCl solution. It can be precipitated quantitatively by concentrations of trichloroacetic acid that do not precipitate the separate components. While the kallikrein inhibitor migrates towards the cathode in paper electrophoresis at pH 7.2, its simplex with heparin does not move under these conditions. In the dissolved simplex, the activity of the kallikrein inhibitor is slightly affected, while the antico-agulation activity of the heparin is markedly reduced.