Completion of the nucleotide sequence of the central region of Tn5confirms the presence of three resistance genes
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (1), 195-205
- https://doi.org/10.1093/nar/13.1.195
Abstract
The DNA sequence of the region located downstream from the kanamycin resistance gene of Tn5 up to the right inverted repeat IS5OR has been determined. This completes the determination of the sequence of Tn5 which is 5818 bp long. The 2.7 Kb central region contains three resistance genes: the kanamycin-neomycin resistance gene, a gene coding for resistance to CL990 an antimitotic-antibiotic compound of the bleomycin family and a third gene that confers streptomycin resistance in some bacterial species but is cryptic in E. coli. A Tn5* mutant able to express streptomycin resistance in E. coli was isolated. With this mutant, it was demonstrated that in E. coli the expression of the three resistance genes is coordinated in a single operon.Keywords
This publication has 24 references indexed in Scilit:
- The inverted repeats of Tn are functionally differentCell, 1980
- A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistanceMolecular Genetics and Genomics, 1979
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Plasmid-Determined Resistance to Antimicrobial AgentsAnnual Review of Microbiology, 1978
- The use of thin acrylamide gels for DNA sequencingFEBS Letters, 1978
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Transposition of R factor genes to bacteriophage lambda.Proceedings of the National Academy of Sciences, 1975
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Proteolytic Cleavage of Native DNA Polymerase into Two Different Catalytic FragmentsEuropean Journal of Biochemistry, 1971
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969