Inactivation of viruses in labile blood derivatives. II. Physical methods

Abstract

The thermal inactivation of viruses in labile blood derivatives was evaluated by addition of marker viruses (VSV, Sinbis, Sendai, EMC) to anti-hemophilic factor (AHF) concentrates. The rate of virus inactivation at 60.degree. C was decerased by at least 100- to 700-fold by inclusion of 2.75 M glycine and 50 percent sucrose, or 3.0 M potassium citrate, additives which contribute to retention of protein biologic activity. Nonetheless, at least 104 infectious units of each virus was inactivated within 10 hours. Increasing the temperature from 60to 70 or 80.degree. C caused a 90 percent or greater loss in AHF activity. An even greater decline in the rate of virus inactivation was observed on heating AHF in the lyophilized state, although no loss in AHF activity was obsrved after 72 hours of heating at 60.degree. C. Several of the proteins present in lyophilized AHF concentrates displayed an altered electrophoretic mobility as a result of exposure to 60.degree. C for 24 hours. Exposure to lyophilized AHF to irradiation from a cobalt 60 source resulted in an acceptable yield of AHF at 1.0, but not at 2.0, megarads. At 1 megarad, greater than or equal to 6.0 logs of VSV and 3.3 logs of Sindbis virus were inactivated.