Phosphatidylinositol 3‐kinase is involved in Toll‐like receptor 4‐mediated cytokine expression in mouse macrophages
Open Access
- 17 February 2003
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 33 (3), 597-605
- https://doi.org/10.1002/eji.200323376
Abstract
Recent evidence suggests a role for phosphatidylinositol (PI) 3-kinase in various inflammatory responses. In this study, the consequences of LPS-induced PI 3-kinase activation on cytokine and chemokine expression and the intracellular mechanisms of inflammatory activation were examined in mouse macrophages. LPS stimulation induced a complex formation between PI 3-kinase and myeloid differentiation factor 88 (MyD88), which was followed by an induction of IL-1β, tumor necrosis factor-α (TNF-α) and macrophage inflammatory protein (MIP)-2. The induction of IL-1β, but not of MIP-2 or TNF-α, was blocked by the PI 3-kinase inhibitors LY294002 and wortmannin. The nuclear factor-κB (NF-κB) inhibitor pyrrolidinedithiocarbamate (PDTC) blocked the induction of IL-1β and TNF-α, but had no effect on MIP-2 expression. Inhibition of PI 3-kinase decreased the LPS-induced transcriptional activity of NF-κB, but it had no effect on the nuclear DNA binding activity of NF-κB. These findings suggest that, while NF-κB nuclear localization and DNA binding are necessary, they are not sufficient for transcriptional activation of the IL-1β gene in the absence of PI 3-kinase activity. Taken together, our results demonstrate that activation of Toll-like receptor (TLR)-4 results in PI 3-kinase-MyD88 complex formation, and that PI 3-kinase activity selectively leads to cytokine induction downstream of TLR4.Keywords
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