DETECTION OF AFRICAN SWINE FEVER VIRUS-ANTIBODIES BY IMMUNOBLOTTING ASSAY
- 1 January 1989
- journal article
- research article
- Vol. 53 (1), 105-107
Abstract
An immunoblotting assay has been adapted to detect antibodies against African swine fever virus. The electrophoretic transfer of proteins and the immunoreaction conditions were optimized, using 4mA/cm2 of current intensity and 10 .mu.g of soluble cytoplasmic antigen of infected cells per strip. Filters of polyvinylidene difluoride showed the highest capacity for protein absorption, but nitrocellulose filters showed lower backgrounds. The specificity and the pattern of the proteins induced by African swine fever virus that reacted with the antisera were determined in immunoblotting assay, IP30 being the most reactive protein.This publication has 7 references indexed in Scilit:
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