The Production of Precipitating Antibody in Chickens to a Substance Present in the Fluids of Nonsecretors of Blood Groups A, B and O

Abstract

Substances have been isolated from the ovarian cyst of a woman of blood group A who is a nonsecretor of A substance, and from the saliva of a man of group B who is a nonsecretor of B substance. Injection of these materials into chickens resulted in the production of precipitating antibody. The antisera exhibited a specificity for the secretions and derivative substances of A, B and O nonsecretors; there was good correlation between Le^a activity of the sample and its ability to precipitate the chicken antisera. It has not, however, proven possible to demonstrate experimentally prepared specific agglutinins for Le(a+) red cells nor can such cells absorb the precipitins from the chicken sera.