Physical map of the seven ribosomal RNA genes of Escherichia coli
Open Access
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (5), 1817-1830
- https://doi.org/10.1093/nar/6.5.1817
Abstract
Escherichia coli DNA was digested with restriction endonucleases BamHI, PstI , EcoRI, SalI , HindIII, XhoI, BglII, SmaI, HpaI and with selected double and triple combinations of the same enzymes. The digests were electrophoresed and hybridized with 32p-labelled ribosomal RNA by using the Southern blotting technique. The resulting bands could be arranged into seven groups, and it was possible to construct a unique physical map of the seven rRNA genes (operons) of the bacterial chromosome. Mapping information obtained on several transducing phages and recombinant plasmids carrying rRNA genes, and mapping data published in the literature helped to determine the final map. The results suggest that phage λ daroE152 carries a “hybridn” rRNA gene which was probably formed by recombination between two different chromosomal rRNA genes.Keywords
This publication has 24 references indexed in Scilit:
- The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methodsFEBS Letters, 1978
- Cloning of an E. coli ribosomal RNA gene and its promoter region fromGene, 1978
- Organization of human δ- and β-globin genes in cellular DNA and the presence of intragenic insertsCell, 1978
- Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.Proceedings of the National Academy of Sciences, 1978
- A new restriction endonuclease from Streptomyces albus GJournal of Molecular Biology, 1978
- A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing λdilv and λdrbs phagesMolecular Genetics and Genomics, 1978
- A physical map of the DNA regions flanking the rabbit β-globin geneCell, 1977
- A rapid procedure for purification of EcoRI endonucleaseBiochemical and Biophysical Research Communications, 1977
- In vitro transcription of the ribosomal RNA genes of E. coli DNAMolecular Genetics and Genomics, 1977
- Isolation of λ transducing phages carrying rRNA genes at the metA—purD region of the Escherichia coli chromosomeFEBS Letters, 1976