Inhibition of enzymes by metal ion-chelating reagents. Theory and new graphical methods of study
- 1 January 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 137 (1), 55-60
- https://doi.org/10.1042/bj1370055
Abstract
1. The mechanism of inhibition of enzymes by metal ion-chelating reagents is discussed and equations derived. 2. Two distinct mechanisms are postulated and graphical methods are given for differentiating between them. 3. Where the metal ion is actually removed from the enzyme to form a co-ordination complex in solution, a procedure is described for obtaining the stability constant for metal–enzyme interaction, the number of metal ions involved and the stoicheiometry of metal ion–ligand interaction.Keywords
This publication has 7 references indexed in Scilit:
- Aspects of Enzyme Mechanisms Studied by Nuclear Spin Relaxation Induced by Paramagnetic ProbesPublished by Wiley ,1970
- The kinetics of enzyme-catalyzed reactions with two or more substrates or products☆III. Prediction of initial velocity and inhibition patterns by inspectionBiochimica et Biophysica Acta, 1963
- ROLE OF ZINC IN ALCOHOL DEHYDROGENASE .5. EFFECT OF METAL-BINDING AGENTS ON THE STRUCTURE OF THE YEAST ALCOHOL DEHYDROGENASE MOLECULE1960
- ROLE OF ZINC IN ALCOHOL DEHYDROGENASES .2. KINETICS OF THE INSTANTANEOUS REVERSIBLE INHIBITION OF YEAST ALCOHOL DEHYDROGENASE BY 1,10-PHENANTHROLINE1958
- ROLE OF ZINC IN ALCOHOL DEHYDROGENASES .3. KINETICS OF A TIME-DEPENDENT INHIBITION OF YEAST ALCOHOL DEHYDROGENASE BY 1,10-PHENANTHROLINE1958
- KINETIC STUDIES ON THE RÔLE OF ZINC AND DIPHOSPHOPYRIDINE NUCLEOTIDE IN THE ACTIVITY OF YEAST ALCOHOL DEHYDROGENASEJournal of Biological Chemistry, 1956
- Analysis of Metal‐Protein ComplexesPublished by Wiley ,1956