MONO-VALENT CATION COMPOSITION AND ATP AND LIPID-CONTENT OF IRREVERSIBLY SICKLED CELLS

Abstract

Discontinuous Stractan gradients were used to separate heterogeneous populations of [human] sickle cells into discrete subpopulations containing varying proportions of reticulocytes, mature discoid cells and irreversibly sickled cells (ISC). The improved homogeneity of these preparations, together with an enhanced yield of ISC, permitted the differentiation of the effects of cell maturation from those of irreversible sickling. With these cell preparations it was possible to define the physical properties of ISC. The marked abnormalities in cation composition of native ISC were confirmed. Measurements of ATP in ISC did not substantiate prior reports of ATP deficits. No evidence for substantial loss of membrane lipids during the process of ISC formation could be demonstrated.