An Electrophysiological Study of the Tentacle-Regulating Potentials in Noctiluca
Open Access
- 1 December 1967
- journal article
- research article
- Published by The Company of Biologists in Journal of Experimental Biology
- Vol. 47 (3), 447-459
- https://doi.org/10.1242/jeb.47.3.447
Abstract
1. The duration and frequency of tentacle-regulating potentials (TRPs) recorded from the vacuole of Noctiluca miliaris were modified by both steady and abrupt changes in the vacuolar potential. The spike frequency increased linearly with an increase in the vacuolar negativity over the range of 0 to -50 mV. 2. A transient decrease of impedance across the soma occurs coincidentally with the negative-going TR spike recorded from the vacuole. Local measurements of impedance changes as well as local surface potential recordings indicate that the TR spike does not propagate over the remainder of the cell from its region of origin near the base of the tentacle. Specimens without visible tentacles exhibited TRPs of normal shape. 3. Concurrent potential recordings from the vacuole and the perinuclear cytoplasm indicate that the vacuolar membrane bordering that portion of the cell is inactive. Except for a relatively more pronounced post-spike hyperpolarization the TRP shape and polarity in the perinuclear cytoplasm, referred to the sea-water bath, are similar to those recorded from the vacuole, but are of smaller amplitude. 4. The TRP appears to consist of a pre-spike pacemaker potential and an action potential having a prolonged post-spike plateau, the pattern recorded from the vacuole being the approximate inverse of that which would be recorded if a microelectrode could be introduced into the cytoplasm adjacent to the electrically active portion of membrane. Several of the findings indicate that the active membrane faces the vacuole and is electrotonically isolated from the perinuclear cytoplasm except through the vacuole.This publication has 16 references indexed in Scilit:
- The Subcellular Origin of Bioluminescence in Noctiluca miliaris The Journal of general physiology, 1967
- Bioelectric Control of Bioluminescence in the Dinoflagellate Noctiluca . I. Specific Nature of Triggering EventsScience, 1965
- Electrical Inexcitability of the Frog Neuromuscular SynapseThe Journal of general physiology, 1963
- Graded and All-or-None Electrogenesis in Arthropod MuscleThe Journal of general physiology, 1961
- Electrophysiological studies of a non‐luminescent form of the dinoflagellate noctiluca miliarisJournal of Cellular and Comparative Physiology, 1960
- AN ANALYSIS OF EXTRACELLULAR POTENTIALS FROM SINGLE NEURONS IN THE LATERAL GENICULATE NUCLEUS OF THE CATThe Journal of general physiology, 1958
- Membrane resting and action potentials from a protozoan, Noctiluca scintillansJournal of Cellular and Comparative Physiology, 1957
- DEMONSTRATION OF TWO STABLE POTENTIAL STATES IN THE SQUID GIANT AXON UNDER TETRAETHYLAMMONIUM CHLORIDEThe Journal of general physiology, 1957
- VAGAL AND SYMPATHETIC EFFECTS ON THE PACEMAKER FIBERS IN THE SINUS VENOSUS OF THE HEARTThe Journal of general physiology, 1956
- PROCESSES OF EXCITATION IN THE DENDRITES AND IN THE SOMA OF SINGLE ISOLATED SENSORY NERVE CELLS OF THE LOBSTER AND CRAYFISHThe Journal of general physiology, 1955