A highly sensitive one-step method for silver intensification of the nickel-diaminobenzidine endproduct of peroxidase reaction.
Open Access
- 1 October 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 37 (10), 1563-1565
- https://doi.org/10.1177/37.10.2674275
Abstract
We have developed a new technique which makes silver intensification of the oxidatively polymerized diaminobenzidine (DAB), the endproduct of peroxidase reaction, less laborious without any loss in selectivity or sensitivity. The new technique is based on two strategies: (a) increasing the argyrophilia of the DAB by modifying its polymerization with Ni ions, and (b) decreasing tissue argyrophila by using a mildly acidic physical developer instead of the alkaline one previously presented. Because the nickel modification takes place in the DAB substrate solution, i.e., in the final step of the peroxidase reaction, only one additional step, the physical development, must be carried out if intensification is needed.Keywords
This publication has 2 references indexed in Scilit:
- Copper-H2O2 oxidation strikingly improves silver intensification of the nickel-diaminobenzidine (Ni-DAB) end-product of the peroxidase reaction.Journal of Histochemistry & Cytochemistry, 1988
- A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection.Journal of Histochemistry & Cytochemistry, 1986