Neutrophil activation by Helicobacter pylori.
Open Access
- 1 August 1991
- Vol. 32 (8), 853-857
- https://doi.org/10.1136/gut.32.8.853
Abstract
Helicobacter pylori infection of the stomach is accompanied by a persistent polymorphonuclear leukocyte (PMNL) infiltrate of the mucosa. The aim of this work was to study the activation of human PMNL by substances produced by H pylori. Filtered H pylori conditioned media stimulated a significant PMNL oxidative burst (p less than 0.002). This was equal to 26% of the maximal response stimulated by the PMNL chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP, 1 mumol/l). The response to FMLP was prolonged by the combined presence of complement inactivated human anti-H pylori plasma and conditioned medium (p less than 0.002). High pressure liquid chromatography of an extract of conditioned medium showed a fraction that stimulated PMNL, eluted, and antigenically cross reacted with FMLP. Washed H pylori cells, and those opsonised with complement inactivated human anti-H pylori plasma, did not induce a significant oxidative burst. Opsonized H pylori, however, prolonged the oxidative burst induced by FMLP (p less than 0.02). In conclusion, H pylori synthesizes and secretes a substance, probably FMLP, that may account for the PMNL accumulation that accompanies H pylori infections. Immune complexes composed of H pylori antigen and specific antibody potentiate the PMNL oxidative burst. This combination of H pylori derived products, and host PMNL and antibodies, may be involved in the mucosal damage observed in H pylori associated gastritis.Keywords
This publication has 26 references indexed in Scilit:
- Hypothesis: Helicobacter pylori, urease, mucus, and gastric ulcerThe Lancet, 1990
- Purification and amino acid sequencing of naturally occurring N-formyl-methionyl oligopeptides fromEscherichia coliCellular and Molecular Life Sciences, 1989
- Opsonophagocytosis of Campylobacter pyloriJournal of Medical Microbiology, 1988
- Production of Peptides Inducing Chemotaxis and Lysosomal Enzyme Release in Human Neutrophils by Intestinal Bacteria in Vitro and in VivoScandinavian Journal of Gastroenterology, 1987
- Interaction of Campylobacter pyloridis with human immune defence mechanismsJournal of Medical Microbiology, 1987
- Enzyme-Linked Immunosorbent Assay for Campylobacter pyloridis: Correlation with Presence of C. pyloridis in the Gastric MucosaThe Journal of Infectious Diseases, 1987
- Local immune response to gastric Campylobacter in non-ulcer dyspepsia.Journal of Clinical Pathology, 1986
- Campylobacter like organisms on the gastric mucosa: culture, histological, and serological studies.Journal of Clinical Pathology, 1984
- Purification and characterization of neutrophil chemotactic factors of Streptococcus sanguisBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- SEROTYPING FOR HOMOTRANSPLANTATION. X. SURVIVAL OF 196 GRAFTED KIDNEYS SUBSEQUENT TO TYPINGTransplantation, 1967