Regulation of Two Carotenoid Biosynthesis Genes Coding for Phytoene Synthase and Carotenoid Hydroxylase during Stress-Induced Astaxanthin Formation in the Green Alga Haematococcus pluvialis
- 1 February 2001
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 125 (2), 810-817
- https://doi.org/10.1104/pp.125.2.810
Abstract
Astaxanthin is a high-value carotenoid used as a pigmentation source in fish aquaculture. In addition, a beneficial role of astaxanthin as a food supplement for humans is becoming evident. The unicellular green alga Haematococcus pluvialis seems to be a suitable source for natural astaxanthin. Astaxanthin accumulation in H. pluvialis occurs in response to environmental stress such as high light and salt stress. Here, the isolation of the H. pluvialis carotenoid biosynthesis gene phytoene synthase is reported. Furthermore, the expression of phytoene synthase and carotenoid hydroxylase, two key enzymes in astaxanthin biosynthesis, was investigated at the transcriptional level. The application of environmental stress resulted in increased steady-state mRNA levels of both genes. High-light intensity led to a transient increase in carotenoid hydroxylase mRNA followed by moderate astaxanthin accumulation. In contrast, salt stress in combination with high light resulted in a sustained increase in both transcripts. The addition of compounds inducing reactive oxygen species did not influence transcript levels of phytoene synthase and carotenoid hydroxylase. The application of an inhibitor of photosynthesis, 3-(3, 4-dichlorophenyl)-1,1-dimethylurea, indicated that the light-induced expression of these carotenoid biosynthesis genes may be under photosynthetic control.Keywords
This publication has 33 references indexed in Scilit:
- Carotenogenesis in the green alga Haematococcus pluvialis: Cellular physiology and stress responsePhysiologia Plantarum, 2000
- Accumulation of secondary carotenoids in flagellates of Haematococcus pluvialis (Chlorophyta) is accompanied by an increase in per unit chlorophyll productivity of photosynthesisEuropean Journal of Phycology, 2000
- GENES AND ENZYMES OF CAROTENOID BIOSYNTHESIS IN PLANTSAnnual Review of Plant Physiology and Plant Molecular Biology, 1998
- Secondary carotenoid accumulation in flagellates of the green algaHaematococcus lacustrisEuropean Journal of Phycology, 1997
- Antioxidant role of astaxanthin in the green alga Haematococcus pluvialisApplied Microbiology and Biotechnology, 1997
- Secondary carotenoid accumulation in flagellates of the green alga Haematococcus lacustrisEuropean Journal of Phycology, 1997
- Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialisFEMS Microbiology Letters, 1996
- Cloning and expression inEscherichia coli of the gene encoding β‐C‐4‐oxygenase, that converts β‐carotene to the ketocarotenoid canthaxanthin inHaematococcus pluvialisFEBS Letters, 1995
- Isolation of a carotenoid biosynthesis gene coding for ζ-carotene desaturase from Anabaena PCC 7120 by heterologous complementationFEMS Microbiology Letters, 1993
- Do carotenoids play a photoprotective role in the cytoplasm of Haematococcus lacustris (Chlorophyta)?Phycologia, 1991