Cryo-Immunology: A Method of Immunization to Autologous Tissue.

Abstract
Freezing of the coagulating gland and seminal vesicle of rabbit, by means of a liquid nitrogen probe, resulted in the formation of antibodies. Tanned cell hemagglutination revealed the presence of antibodies in 6 out of 7 rabbits exposed to freezing. Titers as high as 1:4096 were obtained. No antibody was detected in control animals.