Ion Funnel Trap Interface for Orthogonal Time-of-Flight Mass Spectrometry
- 13 September 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (20), 7845-7852
- https://doi.org/10.1021/ac071091m
Abstract
A combined electrodynamic ion funnel and ion trap coupled to an orthogonal acceleration (oa)-time-of-flight mass spectrometer was developed and characterized. The ion trap was incorporated through the use of added terminal electrodynamic ion funnel electrodes enabling control over the axial dc gradient in the trap section. The ion trap operates efficiently at a pressure of ∼1 Torr, and measurements indicate a maximum charge capacity of ∼3 × 107 charges. An order of magnitude increase in sensitivity was observed in the analysis of low concentration peptides mixtures with orthogonal acceleration (oa)-time-of-flight mass spectrometry (oa-TOF MS) in the trapping mode as compared to the continuous regime. A signal increase in the trapping mode was accompanied by reduction in the chemical background, due to more efficient desolvation of, for example, solvent related clusters. Controlling the ion trap ejection time was found to result in efficient removal of singly charged species and improving signal-to-noise ratio (S/N) for the multiply charged analytes.Keywords
This publication has 27 references indexed in Scilit:
- Charge state separation for protein applications using a quadrupole time-of-flight mass spectrometerRapid Communications in Mass Spectrometry, 2003
- A combined linear ion trap time‐of‐flight system with improved performance and MSn capabilitiesRapid Communications in Mass Spectrometry, 2001
- High-Resolution Electrospray Ionization Orthogonal-Injection Time-of-Flight Mass SpectrometerEuropean Journal of Mass Spectrometry, 2000
- Design and Implementation of a New Electrodynamic Ion FunnelAnalytical Chemistry, 2000
- Peer Reviewed: Orthogonal-Injection TOFMS for Analyzing Biomolecules.Analytical Chemistry, 1999
- High Sensitivity Collisionally-activated Decomposition Tandem Mass Spectrometry on a Novel Quadrupole/Orthogonal-acceleration Time-of-flight Mass SpectrometerRapid Communications in Mass Spectrometry, 1996
- Reflecting time-of-flight mass spectrometer with an electrospray ion source and orthogonal extractionAnalytical Chemistry, 1994
- Electrospray-ionization time-of-flight mass spectrometry in protein chemistryAnalytical Chemistry, 1994
- Detection of electrospray ionization using a quadrupole ion trap storage/reflectron time-of-flight mass spectrometerAnalytical Chemistry, 1993
- Capillary supercritical fluid chromatography at pressures above 400 atmospheresAnalytical Chemistry, 1990