Monoclonal antibodies distinguish identifiable neurones in the leech

Abstract
Monoclonal antibodies were isolated by screening 475 hybridomas obtained from mice immunized with whole leech nerve cords. The majority (about 300) reacted with leech nervous tissue, but only about 40 made antibodies that identified single kinds or small sets of cells. Twenty of the antibodies which react with specific neurons were studied in greater detail and are described. They include antibodies against identified sensory neurons and motor neurons as well as against numerous unidentified cells.