Nucleotide sequence of clonedcDNA specific for rat ribosomal protein L35a

Abstract

A cDNA clone specific for rat ribosomal protein L35a, which is known to be a tRNA binding protein, was isolated by hybrid-selected translation from a cDNA library made for 8-9-S poly(A)-rich RNA from regenerating rat liver. The nucleotide sequence of the cDNA was determined. It consists of one base pair from the 5'' leading sequence, the entire coding sequence of 333 base pairs and 14 base pairs from the 3'' trailing sequence. The primary structure of protein L35a was deduced from the nucleotide sequence. It consists of 109 amino acids with a molecular mass of 12422. The calculated amino acid composition is consistent with that reported for the hydrolysate of L35a. The amino acid sequence showed marked homology with the reported partial sequence of Xenopus leavis ribosomal protein L32, but not significant homology with Escherichia coli ribosomal proteins that bind to tRNA.