High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.
- 1 February 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (3), 669-673
- https://doi.org/10.1073/pnas.83.3.669
Abstract
Purified natural and biosynthetic (recombinant) human granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulate colony formation by myeloid progenitor cells and enhance the function of mature neutrophils. Both of these actions occur at concentrations between 1 and 100 pM, with half-maximal stimulation at 10-20 pM. We have examined specific binding of 125I-labeled GM-CSF to responsive target cells in this range of concentrations. The results show a low number (50-250) of high-affinity (15-30 pM) binding sites on GM-CSF-responsive leukemic cells (KG-1, HL-60), as well as on peripheral blood neutrophils from normal donors. This high-affinity binding component was absent from unresponsive cell lines (KG-1a, K562). These results suggest that this binding site mediates the biological activities of GM-CSF on both proliferation and function of myeloid cells.Keywords
This publication has 18 references indexed in Scilit:
- Human GM-CSF: Molecular Cloning of the Complementary DNA and Purification of the Natural and Recombinant ProteinsScience, 1985
- Human granulocyte-macrophage colony-stimulating factor is a neutrophil activatorNature, 1985
- Purified Human Granulocyte-Macrophage Colony-Stimulating Factor: Direct Action on NeutrophilsScience, 1984
- Binding of the differentiation-inducer, granulocyte-colony-stimulating factor, to responsive but not unresponsive leukemic cell lines.Proceedings of the National Academy of Sciences, 1984
- Iodination of biological samples without loss of functional activityAnalytical Biochemistry, 1982
- Distribution of cells bearing receptors for a colony-stimulating factor (CSF-1) in murine tissues.The Journal of cell biology, 1981
- Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells.The Journal of cell biology, 1980
- Acute Myelogenous Leukemia: A Human Cell Line Responsive to Colony-Stimulating ActivityScience, 1978
- Continuous growth and differentiation of human myeloid leukaemic cells in suspension cultureNature, 1977
- Scatchard plots: Common errors in correction and interpretationSteroids, 1975