Effect of Bordetella pertussis on α1and β-Adrenergic and Cholinergic Muscarinic Receptors in Guinea Pig Lung Membranes

Abstract

After intraperitoneal injection of Bordetella pertussis vaccine to guinea pigs, the α₁- and β-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, ³H-prazosin, 1-³H-dihydroalprenolol and 1-³H-quinuclidinyl benzilate, respectively. Injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (B_max) of ³H-prazosin, while there was about a 30% reduction in 1-³H-dihydroalprenolol binding to β-adrenergic receptors. No difference was observed in the dissociation constant (K_D) for binding of each ligand to α₁- and β-adrenergic receptors. 1-³H-quinuclidinyl benzilate binding indicated that the B_max and K_D for cholinergic muscarinic receptor in pertussis-sensitized guinea pigs were not significantly different from those normal control animals.