Potential inhibitors of L-asparagine biosynthesis. 5. Electrophilic amide analogs of (S)-2,3-diaminopropionic acid

Abstract
Three electrophilic amide analogs of (S)-2,3-diaminopropionic acid (DAP) were prepared as potential inhibitors of L-asparagine synthetase (ASase, from [rat] Novikoff hepatoma, EC 6.3.5.4): (S)-2-amino-3-(2-bromoacetamido)propionic acid hydrobromide (7a), (S)-2-amino-3-(2,2-dichloroacetamido)propionic acid (7b) and ethyl N-[(S)-2-amino-2-carboxyethyl]fumarate (7c). The inhibition of ASase by 7a-c at 1 mM was 93, 19, and 37%, respectively. Compounds 7a-c failed to increase the life span of mice infected with B16 melanoma.